Freshman Jennifer Millington netted a penalty kick from 12 yards out at the 106-minute mark to break a scoreless tie and send the Lady Bulldogs into their first-ever district title game.
"It's a huge win for us to upset the tournament favorite," said Sikeston coach Derrick Long. "Plus we haven't had a big win over a good team in a while, so it was nice to put everything together today."
Although outshot 32-15, the Bulldogs forced tough shots on the Squaws.
"We kept most of their shots from about 30 (yards) out," said Long. "Our goalie didn't have to make a lot of hard saves and that was good for us, because they've got a really good shooting team."
Sikeston's goalkeeper Audra Wooley recorded the shutout with 32 saves.
A key for the Sikeston defense was the play of its midfield defenders, Paige Silverthorn and Megan Underhill.
Said Long, "They couldn't penetrate our defense. Our midfielders controlled the center of the field and, when you do that, you've got a chance to win."
The victory also marked the Sikeston coach's first win over twin brother Eric, St. Vincent's coach, in eight tries.
In the other semifinal, Cape Girardeau Notre Dame, the third seed, shut out No. 2 Perryville 3-0.
Sikeston and Notre Dame play for the district crown at 6 p.m. today at the Sikeston Sports Complex.